Shiga-like Toxin (Stx) (Subunit A) antibody

Catalog No. ABIN2859493

This Mouse Monoclonal antibody specifically detects in EIA and Neut. It exhibits reactivity toward E. coli.

Quick Overview for Shiga-like Toxin (Stx) (Subunit A) antibody (ABIN2859493)

Target: Shiga-like Toxin (Stx)

Reactivity: E. coli

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Enzyme Immunoassay (EIA), Neutralization (Neut)

Clone: 557

Product Details

Binding Specificity: Subunit A

Purification: Protein A affinity chromatography

Purity: > 90 % pure

Isotype: IgG2

Application Details

Application Notes: ELISA. Neutralization.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL ( OD280nm, E0.1% = 1.4)

Buffer: 0.01 M PBS, pH 7.2, containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon receipt, aliquot and store at -20 °C.

Target Details

Target: Shiga-like Toxin (Stx)

Background: Verotoxins are produced by certain enterohaemorrhagic strains of Escherichia coli. Serotype O157:H7 has been the strain most commonly associated with large food bourne outbreaks and has been implicated in a wide spectrum of diseases including blood diarrhoea, haemorrhagic colitis and haemolytic uraemic syndrome. The main sources of infection are contaminated foods of animal origin such as meat and dairy products. Verotoxins, also called Shiga like toxins( SLT) because of their similarity to toxins produced by Shigella dysenteriae, are classified into two main categories: verotoxin 1 (VT1, SLT1, Stx1) and 2 (VT2, SLT2, Stx2). Verotoxin production is the most common criteria for the detection of this group of bacteria rather than tests for the cytopathic effect on Vero cells that gave the toxins their name. Verotoxins contain a single A subunit and multiple copies of a B subunit. The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits.Synonyms: SLT-2 A subunit, SLT-2a, SLT-IIa, Verocytotoxin 2 subunit A, Verotoxin 2 subunit A, rRNA N-glycosidase 2, stx2A, stxA2

Gene ID: 10730

UniProt: P09385